Agar, a heterogeneous polymer of galactose, is the main component of the cell wall of marine red algae. It is well established as a safe, non-digestible carbohydrate in oriental countries. Neoagarooligosaccharides (NAOs) prepared by hydrolyzing agar by microbial β-agarase have been reported to show safety. However, their immunological effects have not been reported yet. Thus, the objective of this study was to investigate immune enhancing effects of neoagarooligosaccharides (NAOs) from marine red algae Gelidium elegans in mice by performing ex vivo experiments. Six-week-old mice were fed ad libitum. NAOs were orally administrated at three different concentrations (100, 500, and 2,500 mg/kg B.W./day) twice a week for four weeks. The group fed with NAOs at 2,500 mg/kg showed the highest proliferation of splenocytes and production levels of cytokines (IL-1β, IL-6, TNF-α) in the ex vivo experiment. In conclusion, NAOs can enhance immune function, increase proliferation of splenocytes, and increase cytokine production by activating macrophages in mice.

Abstract
서 론
재료 및 방법
    1. 시료 추출 및 실험동물
    2. 시약 및 배지
    3. 네오아가로올리고당(NAOs)의 투여
    4. 비장세포 분리
    5. 비장세포 증식능 측정
    6. 복강 대식세포의 분리 및 배양
    7. 마우스 복강 대식세포의 사이토카인(IL-1β, IL-6, TNF-α)생성능
    8. 통계분석
결과 및 고찰
    1. 네오아가로올리고당(NAOs)이 마우스 비장세포 증식능에 미치는 영향
    2. 네오아가로올리고당(NAOs)의 경구 투여후 마우스 복강 대식세포에서 사이토카인 분비능
요약 및 결론
References

• 김경운(㈜온코인사이트 연구소장) | Kyoung-Woon Kim (Director of R&D Center, OncoInsight, Seoul 06349, Korea)
• 원지연(㈜온코인사이트 전임연구원) | Ji-yeon Won (Associate Researcher, OncoInsight, Seoul 06349, Korea)
• 김은주(다인바이오㈜ 연구소장) | Eun Joo Kim (Director of R&D Center, Dyne Bio Inc., Seongnam 13209, Korea)
• 이제현(다인바이오㈜ 대표이사) | Je-Hyeon Lee (CEO, Dyne Bio Inc., Seongnam 13209, Korea)
• 이미연(㈜온코인사이트 부사장) | Miyeon Lee (Vice President, OncoInsight, Seoul 06349, Korea)
• 나득채(㈜온코인사이트 대표이사) | Deukchae Na (CEO, OncoInsight, Seoul 06349, Korea) Corresponding author