Although somatic cell nuclear transfer (SCNT)-derived embryonic stem cells (ESCs) in pigs have great potential, their use is limited because the establishment efficiency of ESCs is extremely low. Accordingly, we tried to develop in-vitro culture system stimulating production of SCNT blastocysts with high performance in the colony formation and formation of colonies derived from SCNT blastocysts for enhancing production efficiency of porcine ESCs. For these, SCNT blastocysts produced in various types of embryo culture medium were cultured in different ESC culture medium and optimal culture medium was determined by comparing colony formation efficiency. As the results, ICM of porcine SCNT blastocysts produced through sequential culture of porcine SCNT embryos in the modified porcine zygote medium (PZM)-5 and the PZM-5F showed the best formation efficiency of colonies in α-MEM-based medium. In conclusion, appropriate combination of the embryo culture medium and ESC culture medium will greatly contribute to successful establishment of ESCs derived from SCNT embryos.

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
CONFLICTS OF INTEREST
ACKNOWLEDGEMENTS
ORCID
REFERENCES

• Na Rae Han(Department of Animal Life Science, Kangwon National University, Chuncheon 24341, Korea)
• Song Baek(Department of Animal Life Science, Kangwon National University, Chuncheon 24341, Korea)
• Yongjin Lee(College of Veterinary Medicine, Kangwon National University, Chuncheon 24341, Korea)
• Joohyeong Lee(Institute of Veterinary Medicine, Kangwon National University, Chuncheon 24341, Korea)
• Jung Im Yun(Institute of Animal Resources, Kangwon National University, Chuncheon 24341, Korea)
• Eunsong Lee(College of Veterinary Medicine, Kangwon National University, Chuncheon 24341, Ko)
• Seung Tae Lee(Department of Animal Life Science, Kangwon National University, Chuncheon 24341, Korea, Department of Applied Animal Science, Kangwon National University, Chuncheon 24341, Korea)