Nitric oxide (NO)-induced protein S-nitrosylation triggers mitochondrial dysfunction and was related to cell senescence. However, the exact mechanism of these damages is not clear. In the present study, to investigate the relationship between in vitro aging and NO-induced protein S-nitrosylation, oocytes were treated with sodium nitroprusside dihydrate (SNP), and the resultant S-nitrosylated proteins were detected through biotin-switch assay. The results showed that levels of protein S-nitroso thiols (SNO)s and expression of S-nitrosoglutathione reductase (GSNOR) increased, while activity and function of mitochondria were impaired during oocyte aging. Addition of SNP, a NO donor, to the oocyte culture led to accelerated oocyte aging, increased mitochondrial dysfunction and damage, apoptosis, ATP deficiency, and enhanced ROS production. These results suggested that the increased NO signal during oocyte aging in vitro, accelerated oocyte degradation due to increased protein S-nitrosylation, and ROS-related redox signaling.

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
CONFLICTS OF INTEREST
ACKNOWLEDGEMENTS
AUTHOR CONTRIBUTIONS
AUTHOR’S POSITION AND ORCID NO
REFERENCES

• Ying-Jie Niu(Department of Animal Science, Chungbuk National University, Cheongju 28644, Korea)
• Dongjie Zhou(Department of Animal Science, Chungbuk National University, Cheongju 28644, Korea)
• Wenjun Zhou(Department of Animal Science, Chungbuk National University, Cheongju 28644, Korea)
• Zheng-Wen Nie(Department of Animal Science, Chungbuk National University, Cheongju 28644, Korea)
• Ju-Yeon Kim(Department of Animal Science, Chungbuk National University, Cheongju 28644, Korea)
• YoungJin Oh(Chung Cheong Buk-Do Institute of Livestock and Veterinary Research, Cheongju 28153, Korea)
• So-Rim Lee(Chung Cheong Buk-Do Institute of Livestock and Veterinary Research, Cheongju 28153, Korea)
• Xiang-Shun Cui(Department of Animal Science, Chungbuk National University, Cheongju 28644, Korea) Corresponding author