Preserving intact genetic material and delivering it to the next generation are the most significant tasks of living organisms. The integrity of DNA sequences is under constant threat from endogenous and exogenous factors. The accumulation of damaged or incompletely-repaired DNA can cause serious problems in cells, including cell death or cancer development. Various DNA damage detection systems and repair mechanisms have evolved at the cellular level. Although the mechanisms of these responses have been extensively studied, the global RNA expression profiles associated with genomic instability are not well-known. To detect global gene expression changes under different DNA damage and hypoxic conditions, we performed RNA-seq after treating human cervical cancer cells with ionizing radiation (IR), hydroxyurea, mitomycin C (MMC), or 1% O2 (hypoxia). Results showed that the expression of 184–1037 genes was altered by each stimulus. We found that the expression of 51 genes changed under IR, MMC, and hypoxia. These findings revealed damage-specific genes that varied differently according to each stimulus and common genes that are universally altered in genetic instability.

Introduction
Materials and Methods
    1. 세포 배양 및 DNA 상해반응
    2. 4-thiouridine 처리와 RNA 정제 및 total RNA 라이브러리구축
    3. Total RNA 라이브러리 구축 및 RNA-seq data 분석
    4. Kyoto Encyclopedia of Genes and Genomes (KEGG)pathway 분석 및 gene ontology (GO) 분석
Results
    1. 유전자 발현 패턴 프로파일 분석
    2. GO term과 KEGG 경로 분석
    3. 자극에 공통적으로 변화하는 유전자
Discussion
Acknowledgements
Conflicts of Interest
References

• Jung-Min Oh(Department of Oral Biochemistry, Dental and Life Science Institute, School of Dentistry, Pusan National University, Yangsan 50612, Republic of Korea) Correspondence author