The objective of the present study was to investigate the molecular mechanisms involved in the activity of [10]-gingerol using A2058 human melanoma cells. [10]-Gingerol inhibited the proliferation of A2058 cells by 50% at a concentration of 52 μM. Such inhibition was dose-dependent accompanied by morphological change indicative of apoptosis. Furthermore, flow cytometric analysis by Annexin V and PI double staining showed that [10]-gingerol increased the extent of apoptosis. Analysis of the mechanism of these events indicated that [10]-gingerol increased the ratio of Bax to Bcl-2, resulting in the activation of caspase-9, caspase-3, and poly-ADP-ribose polymerase in a dose-dependent manner.

Abstract
Introduction
Materials and Methods
    1. Samples
    2. Materials
    3. Cell culture and cell viability assay
    4. Nuclear staining with Hoechst 33258
    5. Apoptosis analysis
    6. Western blotting analysis
    7. Statistical analysis
Results and Discussion
    1. Inhibitory effects of [10]-gingerol in A2058 cells
    2. Induction of apoptosis by [10]-gingerol in A2058 cells
    3. Effects of [10]-gingerol on apoptosis in A2058 cells
    4. Effects of [10]-gingerol on the expression of Bcl-2family and caspase protein
    5. Effects of [10]-gingerol on the expression of MAPK
Conclusion
References

• Tae Eun Guon(Graduate Student, Dept. of Food and Nutrition, Duksung Women’s University, Seoul 01369, Korea)
• Ha Sook Chung(Professor, Dept. of Food and Nutrition, Duksung Women’s University, Seoul 01369, Korea) Corresponding Author