The purpose of this study was to investigate the biological activity of fucoidan, a sulfur-containing polysaccharide, on cytotoxicity and apoptosis in the human HT-29 colorectal cancer cell line using cell viability, Flow cytometry, Western blot, and RT-PCR analyses. Fucoidan inhibited the proliferation of HT-29 cells by 39.6% at a concentration of 100 μg/mL for 72 h. The inhibition was dose-dependent and accompanied by apoptosis. Flow cytometric analysis showed that fucoidan increased early apoptosis and late apoptosis by 65.84% and 72.09% at concentrations of 25 and 100 μg/mL, respectively. Analysis of the mechanism of these events indicated that fucoidan-treated cells exhibited increases in the activation of caspase-3, caspase-8, and PARP in a dose-dependent manner. These results suggest that fucoidan may inhibit the growth of human colorectal cancer cells by various apoptosis-promoting effects, as well as by apoptosis itself.

Abstract
서 론
재료 및 방법
    1. 실험재료
    2. 세포주 및 세포 배양
    3. 세포 독성 실험(MTT assay)
    4. 유세포 분석(Flow cytometry analysis)
    5. 단백질 발현 실험(Western blott analysis)
    6. mRNA 발현 실험(RT-PCR)
    7. 통계처리
결과 및 고찰
    1. HT-29 세포 증식 변화
    2. HT-29 세포사멸 유도
    3. Caspase-3 및 caspase-8의 활성화
    4. Bcl-2, Bax 및 p53 발현
    5. Apoptosis 관련 mRNA 발현
요약 및 결론
References

• 김민지(덕성여자대학교 과학기술대학 식품영양학과 석사과정생) | Min Ji Kim (Graduate Student, Dept. of Food and Nutrition, College of Science and Technology, Duksung Women’s University, Seoul 01369, Korea)
• 정하숙(덕성여자대학교 과학기술대학 식품영양학과 교수) | Ha Sook Chung (Professor, Dept. of Food and Nutrition, College of Science and Technology, Duksung Women’s University, Seoul 01369, Korea) Corresponding Author