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Comparative mitogenomics of Pleurotus ostreatus Gonji7ho and its cap color mutant KCI 등재
- 언어ENG
- URLhttps://db.koreascholar.com/Article/Detail/415251
pp.43-49
Pleurotus ostreatus is a globally cultivated mushroom crop. Cap color is a quality factor in P. ostreatus. However, cap color can spontaneously mutate, degrading the quality of the mushroom on the market. Early detection and removal of mutant strains is the best way to maintain the commercial value of the crop. To detect the cap color mutant Gonji7ho, molecular markers were developed based on insertion/deletions (InDels) derived from the comparison of mitogenomes of Gonji7ho and Gonji7hoM mushrooms. Sequencing, assembly, and comparative analysis of the two mitogenomes revealed genome sizes of 73,212 bp and 72,576 bp with 61 and 57 genes or open reading frames (ORFs) in P. ostreatus Gonji7ho and Gonji7hoM, respectively. Fourteen core protein-encoding genes, two rRNA, and 24 tRNA with some OFRs were predicted. Of the 61 genes or OFRs in the wild type, dpo, rpo, and two orf139 were missing (or remnant) in the mutant strain. Molecular markers were developed based on the sequence variations (InDels) between the two mitogenomes. Six polymorphic molecular markers could detect the mutated mitochondria by PCR. These results provide basic knowledge of the mitogenomes of wild-type and mutant P. ostreatus, and can be applied to discriminate mutated mitochondria.
INTRODUCTION
MATERIALS AND METHODS
Fungal strains and growth conditions
Fructification and phenotypic evaluation
Genomic DNA sequencing and de novo assembly
RESULTS AND DISCUSSION
Phenotypic differences between Gonji7ho and its capcolor mutant Gonji7hoM
Mitogenome structure and organization
Gene content and order
Sequence variation and detection of mutatedmitochondria by molecular markers
Acknowledgments
REFERENCES
