Human intestinal flora is very diverse; with lactic acid bacteria (LAB) existing as part of the most vital gut microbes that improve host health. The application of LAB as a whole organism or its metabolite in the case of probiotic and bacteriocin respectively is extensive. Thus, the need to always bio-prospect for newer strains of LAB is essential. This study focused on isolating LAB from kunun-zaki and kindirmo, a fermented non-alcoholic beverage of non-dairy and dairy sources respectively, explored their physiological and biochemical properties, antibiotics sensitivity pattern and identified based on their 16S rRNA sequencing. A total of eighty isolates were selected sixty-six from kunun-zaki and fourteen from kindirmo in which 93.7% were bacilli and 6.3% were spherical in shape having 68.75% and 30% homofermentative and heterofermentative pathway respectively. All isolates have the ability to utilize glucose to produce lactic acid while their tolerance to pH 3 and salt concentration at 2%, 4% and 6.5% varied widely. Thirty-four isolates based on their physiological and biochemical properties were selected for molecular identification to ascertain their genera and species. Limosilactobacillus fermentum (68%); Lactiplantibacillus plantarum (6%) and Weissella confusa (3%) were confirmed species isolated. Thus, it was concluded that traditional fermented foods such as kunun-zaki and kindirmo are a good source to bio-prospect for LAB for product development, starter culture and probiotic study.

Abstract
INTRODUCTION
MATERIALS AND METHODS
    Collection of Samples
    Isolation of Lactic Acid Bacteria
    Characterization of Bacterial Isolates
    Long Term Storage and Revival of Isolates
    Physiological and Biochemical Characterization of theIsolated Lactic Acid Bacteria
    Antibiotic Resistant Pattern of the Isolated Lactic AcidBacteria
    Molecular Identification of the Isolated Lactic AcidBacteria
RESULTS
    Characterization of the Lactic Acid Bacteria
    Biochemical Characterization of the Isolated Lactic AcidBacteria
    Antibiotic Resistance Pattern of the Isolated Lactic AcidBacteria
    Molecular Identification of the Isolated Lactic AcidBacteria
DISCUSSION
CONCLUSION
Acknowledgment
Conflict of Interest
Authors Contribution
References

• Timothy Bamgbose(India Council of Medical Research, National Institute of Malaria Research,Department of Microbiology, Ahmadu Bello University,West Africa Centre for Cell Biology of Infectious Pathogens) Corresponding author
• Swati Sinha(India Council of Medical Research, National Institute of Malaria Research)
• Isa O Abdullahi(Department of Microbiology, Ahmadu Bello University)
• Helen I Inabo(Department of Microbiology, Ahmadu Bello University)
• Mohammed Bello(Department of Veterinary Public Health and Preventive Medicine, Ahmadu Bello University)
• Lokesh D Kori(India Council of Medical Research, National Institute of Malaria Research)
• Elmer N Ametefe(Department of Biochemistry Cell and Molecular Biology, University of Ghana)
• Anupkumar R Anvikar(India Council of Medical Research, National Institute of Malaria Research)