This study aimed to investigate the levels of perception of, knowledge of, and attitude toward patient safety and identify factors that affected patients’ attitude towards patient safety. In this descriptive study, participants included 196 patients hospitalized at a tertiary hospital in South Korea. Data were collected using self-reported questionnaires. Perception of and attitude towards patient safety were measure with a tool developed by Ahn Jin-ok, and the questionnaire for knowledge of patient safety was developed by researchers based on a literature review and validated by an expert group. Data were analyzed with Pearson’s correlation coefficients and multiple regression. The average perception of patient safety was 35.48(±6.80) out of 50; the average knowledge of patient safety was 84.33(±8.66) out of 100; and the average attitude towards patient safety was 36.88(±3.74) out of 50. The perception and knowledge of patient safety, level of hospital safety, and age were identified as influential factors explaining 13% of the variation in attitude towards patient safety. The results showed that the direct and indirect educational experience of patients may increase their perception and knowledge of patient safety that can influence their attitude towards patient safety. Therefore, patient education would be an important intervention to improve patients’ attitude towards patient safety. We recommend further studies with educational interventions for improving patient safety activities.