논문 상세보기
pp.23-31
Lac 색소의 열수와 메탄올 추출물의 일부 유리아미노산과 그 유도체의 조성 및 함량, phenolic acid와 flavonoid의 조성 및 함량을 분석하고, 일부 피부미용 화장품 생리활성을 조사한 결과는 다음과 같다. 유리아미노산은 열수와 메탄올 추출물에서 각각 검출되지 않았으며, 단백질 비구성 아미노산 유도체는 열수 추출물에서 2종, 메탄올 추출물에서는 3종이 검출되었다. Phenolic acid와 flavonoids의 조성 및 함량분석에서, phenolic acid의 경우 열수 추출물에서 2종, 메탄올 추출물에서 5종이 검출되었다. 또한 flavonoids의 경우 열수 추출물에서는 검출되지 않았으나, 메탄올 추출물에서는 rutin과 taxifolin이 약간 존재하는 것으로 나타났다. DPPH radical 소거 활성은 메탄올 추출물이 40.43 ± 0.21%로 열수 추출물에서보다 약간 높게 나타났고, Tyrosinase 저해 활성은 메탄올 추출물이 열수 추출물에서 보다 약 2배 정도 높은 것으로 나타났다. HaCaT 세포주에 대한 세포독성은 모든 처리 농도에서 나타나지 않았으며, 처리 농도가 증가함에 따라 세포 생장이 증가하는 경향이었다.
The composition and content of some free amino acids and non-protein amino acid derivatives of the hot water and methanol extract of the Lac pigment, and the composition and content of phenolic compounds were analyzed, and the physiological activity of some skin beauty cosmetics were as follows. Free amino acids were not detected in hot water and methanol extracts, respectively, 2 kinds of protein non-constituent amino acid derivatives were detected in hot water extract and 3 kinds were detected in methanol extract. In the composition and content analysis of phenolic acid and flavonoids, 2 types of phenolic acid were detected in hot water extract and 5 types were detected in methanol extract. In addition, flavonoids were not detected in hot water extract, but rutin and taxifolin were slightly present in methanol extract. DPPH radical scavenging activity was slightly higher in methanol extract than in hot water extract at 40.43 ± 0.21%, and in tyrosinase inhibitory activity, methanol extract was approximately twice as high as in hot water extract. In addition, cytotoxicity to the HaCaT cell line was not observed at all treatment concentrations, and cell growth tended to increase as the treatment concentration increased. In the treatment concentration of these extracts of 100㎍/ml, the cell activity of about 30-60% was higher than that of the control. Therefore, according to the above results, the hot water and methanol extract of Lac pigment contains some non-protein derivatives, phenolic acid and flavonoid components, and it appears to have some physiological activity for skin beauty. It is believed that it is valuable for use as an ingredient, but it is considered that additional research is needed continuously in order to actually use it.
Abstract
Introduction
Study Methods
1. 실험재료
2. 시료의 제조
3. 유리아미노산 및 유도체 분석
4. Phenolic compounds의 조성 및 함량 분석
5. DPPH에 의한 전자공여능 측정
6. Tyrosinase 저해 활성
7. 세포독성 측정
8. 통계분석
Results
1. 추출물의 수율
2. 추출물의 유리아미노산 분석
3. Phenolic acid와 flavonoids의 조성 및 함량
4. DPPH에 의한 라디칼 소거활성
5. Tyrosinase 활성 저해
6. 세포독성 효과
Conclusion
Author Information
References
