In this paper, cycle performance analysis of two-stage compression two-stage expansion refrigeration system using eco-friendly refrigerants is presented to offer the basic design data for the operating parameters of the system. Eco-friendly refrigerant R600a(Isobutane) for freon refrigerant R22 were used working fluids in this study. The coefficient of performance(COP) of R600a is about 5% greater than that of R22 two-stage compression refrigeration system in the range of evaporation temperature –30℃∼-60℃. The coefficient of performance of two-stage compression and two-stage expansion refrigeration system decreases with the increasing condensation temperature and superheating degree but increases with the increasing evaporation temperature, subcooling degree and mass flow rate ration of intercooler.