This study was conducted to evaluate the effects of ImmunoSEB as an immune-booster additive on the performance of broiler chicks. A total of 1150 broiler chickens were randomly allotted to three treatments on the basis of body weight (10 pens per treatment with 40 broilers in each pen): the control (CON), CON + ImmunoSEB 0.025% in feed (SEB25), and CON + ImmunoSEB 0.050% in feed (SEB50). The experiment was conducted for d 42 in three phases (phase 1, d 0–14; phase 2, d 15–28; and phase 3, d 29–42). There were significant differences in the average daily gain (ADG) and feed intake. The ADG at d 14 in the SEB50 treatment was greater than that in the CON treatment. The overall ADG in the SEB50 treatment was greater than that in the CON treatment. During d 0–14, the feed intake of chickens in the SEB50 treatment increased compared to that in the CON treatment. The crude protein and lysine digestibility improved in the SEB25 and SEB50 treatments compared to those in the CON treatment at d 28. Superoxide dismutase concentration significantly increased in the SEB50 treatment compared to that in the CON treatment. The interleukin-1β and tumor necrosis factor-α concentrations were higher in the cecum of chickens in the CON treatment than in the SEB25 and SEB50 treatments. A lower population of E. coli was detected in the ileum and cecum of broilers fed the SEB50 diet compared to those of broilers fed the CON diet. The overall result showed the beneficial effects of using ImmunoSEB at a dose of 0.050% in broiler chickens.

ABSTRACT
Ⅰ. INTRODUCTION
Ⅱ. MATERIALS AND METHODS
    1. Animals and experimental design
    2. Chemical analysis
    3. Blood metabolites
    4. Antioxidant status
    5. Inflammatory and proinflammatory cytokine determination
    6. Microbial analyses
    7. Small intestinal morphology
    8. Statistical analyses
Ⅲ. RESULTS
    1. Growth performance
    2. Nutrient digestibility
    3. Blood metabolites and antioxidant status
    4. Inflammatory and proinflammatory cytokine levels
    5. Intestinal microbiota
    6. Gut morphology
Ⅳ. DISCUSSION
Ⅴ. REFERENCES

• Anushka Lokhande(강원대학교 동물산업융합학과 대학원생)
• Santosh Ingale(인도 어드벤스드엔자임 팀장)
• 문준영(강원대학교 동물산업융합학과 대학원생) | JunYoung Mun (Graduate Students, Department of Animal Industry Convergence, Kangwon National University, Chuncheon 24341, Korea)
• 김진수(강원대학교 동물산업융합학과 교수) | Jin Soo Kim (Professor, Department of Animal Industry Convergence, Kangwon National University, Chuncheon 24341, Korea) Corresponding author