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Virus Elimination from in Vitro Cultured Cnidium officinale by Thermotherapy and Chemotherapy KCI 등재
pp.145-150
Because Cnidium officinale is susceptible to viral infection, this study investigated the proportion of virus elimination and plant growth after treatments with thermotherapy and chemotherapy. In the thermotherapy group, Apple stem grooving virus (ASGV) and Cnidium vein yellowing virus–2 (CnVYV-2) were significantly reduced in 33℃ treatment. However, Cnidium vein yellowing virus–1 (CnVYV-1) was not affected by any of the four temperature treatments, and Cucumber mosaic virus (CMV) did not show the tendency to eliminate viruses according to temperature treatment. In the investigation of in vitro growth characteristics, plants were not significantly affected by temperature treatments. In the chemotherapy group, 40 mg･L-1 ribavirin treatment was the most effective in eliminating viruses. Different ribavirin concentrations (0 to 40 mg･L-1) did not significantly influence the in vitro plant survival rate. ASGV was completely eliminated with combined thermotherapy and chemotherapy (T29R20 and T29R40). Although the combined thermotherapy and chemotherapy treatment did not affect in vitro plant growth, it significantly influenced root development. The results indicate that thermotherapy, chemotherapy, and the combined treatment effectively eliminated these four viruses (ASGV, CnVYV-1, CnVYV-2, and CMV) without producing any major problems in C. officinale.
Introduction
Materials and Methods
1. Plant materials
2. Chemotherapy
3. Thermotherapy
4. Combined chemotherapy and thermotherapy
5. Virus detection
Results and Discussion
1. Effect of thermotherapy on growth of in vitroplants
2. Effect of chemotherapy on growth of in vitroplants
3. Effect of combined thermotherapy and chemotherapytreatments on growth and proliferation of in vitroplants
Acknowledgement
References
