본 연구에서는 미생물 첨가에 따라 거세한우 비육우 분의 이화 학적 특성, 미생물 성상, 가스발생량 및 퇴비 부숙도에 미치는 영 향을 규명하고자 수행하였다. 이상의 결과를 종합하면, 4주 후,미생물 첨가구에서 수분, 유기물, 총질소 함량 및 pH가 낮았으며, 나머지 이화학적 특성에서는 차이가 나타나지 않았다. 유산균과 효모균 수는 증가하였으며, 대장균 수는 감소하였다. 12주 후, 미 생물 첨가구에서 유산균과 고초균 수는 높았으나, 수분, 효모 및 대장균 수는 낮게 낮았다. 하지만 암모니아, 황화수소 발생량과 퇴비 부숙도는 미생물 첨가에 의한 효과가 나타나지 않았다. 따라 서, 거세한우 분에 미생물을 첨가하면 유익균은 증가하고, 병원성 미생물은 감소하여, 비육우의 생산성은 증진될 것으로 사료되지 만, 가스 발생량 및 퇴비 부숙도에 대한 추가적인 연구는 지속적 으로 수행되어야 할 것으로 사료된다.

The present study investigated effects of microbial additives on the floor of Hanwoo steer manure in barn. The treatment following: without additives (CON); additives (AMA). Each treatment used 3 barns as replication and each barn contained 5 Hanwoos. The Hanwoo steer manure in barns was sub-sampled from 5 sides of pen at 0, 4 and 12 weeks. The sub-samples were used for analyses of chemical compositions, microbial counts, gas emissions and compost maturity. The concentrations of moisture, organic matter, total nitrogen and carbon-to-nitrogen (C/N ratio) of Hanwoo steer manure before the microbial additives were each 59.1%, 83.2%, 1.78% and 50.0%, respectively. The counts of lactic acid bacteria, Yeast, Bacillus subtilis, and Escherichia coli (E. coli) were each 5.94, 6.83, 7,28 and 5.52 cfu/g, but Salmonella was not detected. The ammonia-N gas was 4.67 ppm, but hydrogen sulfide gas was not detected. After 4 weeks, moisture, organic matter, total nitrogen, pH and yeast count were lowest (p<0.05). The lactic acid bacteria, yeast, Escherichia coli (E. coli) and ammonia-N gas were not effects of microbial additives. All treatments was not detected at Salmonella count and hydrogen sulfide emission, and compost maturity was completed. After 12 weeks, the lactic acid bacteria and Bacillus subtilis were highest in AMA, while moisture, yeast and E. coli were lowest (p<0.05). The ammonia-N gas was not effect by microbial additive. Salmonella and hydrogen sulfide emission were not detected in all treatments, and compost maturity was completed. Therefore, in present study, the microbial additive did not affect of gas and compost maturity, but the pathogenic microorganism such as E. coli, were inhibited by microbial additives.

ABSTRACT
Ⅰ. 서론
Ⅱ. 재료 및 방법
    1. 공시 동물과 시험구
    2. 우분의 이화학적 특성
    3. 미생물 성상
    4. 가스 발생량 및 부숙도
    5. 통계처리
Ⅲ. 결과 및 고찰
    1. 미생물 첨가 전 우분의 성상
    2. 미생물 첨가 4주 후 우분의 성상
    3. 미생물 첨가 12주 후 우분의 성상
Ⅳ. 요약
Ⅴ. 사사
Ⅵ. REFERENCES

• 주영호(경상국립대학교 농업생명과학연구원) | Young Ho Joo (Institute of Agriculture and Life Science, Gyeongsang National University)
• 서명지(경상국립대학교 응용생명과학부(BK21 Four)) | Myeong Ji Seo (Division of Applied Life Science (BK21Four), Gyeongsang National University)
• 정승민(경상국립대학교 응용생명과학부(BK21 Four)) | Seung Min Jeong (Division of Applied Life Science (BK21Four), Gyeongsang National University)
• 김지윤(경상국립대학교 응용생명과학부(BK21 Four)) | Ji Yoon Kim (Division of Applied Life Science (BK21Four), Gyeongsang National University)
• 김삼철(경상국립대학교 농업생명과학연구원, 경상국립대학교 응용생명과학부(BK21 Four)) | Sam Churl Kim (Institute of Agriculture and Life Science, Gyeongsang National University, Division of Applied Life Science (BK21Four), Gyeongsang National University) Corresponding author