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섬진강-광양만 하구 기수 재첩 (Corbicular japonica)의 분자 계통유전학적 분석 KCI 등재

Molecular Phylogenetic Analysis of the Brackish Water Clam (Corbicular japonica) from Seomjin River to Gwangyang Bay, South Korea

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  • URLhttps://db.koreascholar.com/Article/Detail/419279
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생태와 환경 (Korean Journal of Ecology and Environment)
초록

An estuary is a water ecosystem with a high abundance of the species diversity, due to a variety of complex physicochemical factors of the area where freshwater and ocean mixed. The identification of Corbicula species in the estuary environments is difficult because of various morphological characteristics. In this study, we provide taxonomic information on Corbicula species with taxonomic difficulties using morphological and genetic analysis. This study was conducted on clams from the Seomjin River-Gwangyang Bay, one of the major production area of marsh clam in Korea. As a result, we characterized Cytocrome C Oxidase subunit I (COI) sequences of the Corbicula. The 636 bp nucleotide sequences of COI have 98% homology among Corbicula species collected from 2 sites of Seomjin River-Gwangyang Bay. The phylogenetic analysis with 17 species of Corbicula indicated that most of the species collected from Seomjin River-Gwangyang Bay were brackish water clam (Corbicula japonica), and only one Asian clam (Corbicula fluminea). The evolutionary distance between C. japonica and C. fluminea was less than 0.003. Therefore, it was confirmed that C. japonica is phylogenetically closely related to C. fluminea. In 9 species of Cyrenidae, phylogenetic tree was classified into three lineages. These results will be used as an important data for an identification of clam species by providing genetic information for Corbicula species with a morphological diversity. Key words: Corbicula japonica, cytochrome c oxidase subunit I (

목차
Abstract
서 론
재료 및 방 법
    1. 생물 채집 정보
    2. 기수재첩 Genomic DNA 추출
    3. 미토콘드리아 DNA의 COI 유전자 PCR 증폭과염기서열 분석
    4. DNA 데이터 분석
결과 및 고 찰
적 요
REFERENCES
저자
  • 김지훈(전남대학교 해양융합과학과) | Ji-Hoon Kim (Department of Ocean Integrated Science, Chonnam National University)
  • 김원석(전남대학교 해양융합과학과) | Won-Seok Kim (Department of Ocean Integrated Science, Chonnam National University)
  • 박기연(전남대학교 수산과학연구소) | Kiyun Park (Fisheries Science Institute, Chonnam National University)
  • 곽인실(전남대학교 해양융합과학과, 전남대학교 수산과학연구소) | Ihn-Sil Kwak (Department of Ocean Integrated Science, Chonnam National University, Fisheries Science Institute, Chonnam National University) Corresponding author