The aim of this study was to investigate the effect of isolated lactic acid bacteria (LAB) on the quality of high moisture rye silage. Rye forage (Secale cereale L.) was harvested at the heading stage (27.3% of dry matter (DM)) and cut into approximately 3-5 cm lengths. Then, the forage divided into 4 treatments with different inoculants: 1) No additives (CON); 2) Lactobacillus brevis strain 100D8 at a 1.2 x 105 colony-forming unit (cfu)/g of fresh forage (LBR); 3) Leuconostoc holzapfelii strain 5H4 at a 1.0 x 105 cfu/g of fresh forage (LHO); and 4) Mixture of LBR and LHO (1:1 ratio) applied at a 1.0 x 105 cfu/g of fresh forage (MIX). About 3 kg of forage from each treatment was ensiled into a 20 L mini-bucket silo in quadruplicate for 100 days. After silo opening, silage was collected for analyses of chemical compositions, in vitro nutrient digestibilities, fermentation characteristics, and microbial enumerations. The CON silage had the highest concentrations of neutral detergent fiber and acid detergent fiber (p = 0.006; p = 0.008) and a lowest in vitro DM digestibility (p < 0.001). The pH was highest in CON silage, while lowest in LBR and MIX silages (p < 0.001). The concentrations of ammonia-N, lactate, and acetate were highest in LBR silage (p = 0.008; p < 0.001; p < 0.001). Propionate and butyrate concentrations were highest in CON silage (p = 0.004; p < 0.001). The LAB and yeast counts were higher in CON and LHO silages compare to LBR and MIX silages (p < 0.001). However, the mold did not detect in all treatments. Therefore, this study could conclude that L. brevis 100D8 and Leu. holzapfelii strain 5H4 can improve the digestibility and anti-fungal activity of high moisture rye silage.

ABSTRACT
Ⅰ. INTRODUCTION
Ⅱ. MATERIALS AND METHODS
    1. Silage production
    2. Chemical compositions and in vitro nutrient digestibility
    3. Fermentation characteristics
    4. Microbial enumerations
    5. Statistical analysis
Ⅲ. RESULTS
Ⅳ. DISCUSSION
Ⅴ. CONCLUSION
Ⅵ. ACKNOWLEDGMENT
Ⅶ. REFERENCES

• Seong Shin Lee(Animal Nutrition and Physiology Division, National Institute of Animal Science, RDA)
• Seung Min Jeong(Division of Applied Life Science (BK21Four), Gyeongsang National University)
• Myeong Ji Seo(Division of Applied Life Science (BK21Four), Gyeongsang National University)
• Young Ho Joo(Institute of Agriculture & Life Science, Gyeongsang National University)
• Dimas Hand Vidya Paradhipta(Department of Animal Nutrition and Feed Science, Faculty of Animal Science, Universitas Gadjah Mada)
• Pil Nam Seong(Animal Nutrition and Physiology Division, National Institute of Animal Science, RDA)
• Sam Churl Kim(Division of Applied Life Science (BK21Four), Gyeongsang National University, Institute of Agriculture & Life Science, Gyeongsang National University) Corresponding author