The aim of this study was to investigate dry matter productivity and nitrate nitrogen content in the growth stages of barnyard millet (Echinochloa esculenta) cultivated for feed, which was treated with different nitrogen fertilization levels. An early variety of barnyard millet (cv. Shirohie) was used for the test, and the different treatments with nitrogen fertilizer were as follows: 50% (N-40 kg/ha, T1), 100% (N-80 kg/ha, reference amount, T2), 150% (N-120 kg/ha, T3), 200% (N- 160 kg/ha, T4), 250% (N-200 kg/ha, T5), and 300% (N-240 kg/ha, T6). Sowing was done on May 13, 2021 and plants were harvested for four stage; vegetative stage, elongation stage, heading stage, and milk stage. The length of the millet increased significantly as the amount of nitrogen fertilization increased during the harvest period (p<0.05), but the difference was insignificant during the milk stage (p>0.05). Moreover, barnyard millet dry matter yield increased significantly as the levels of nitrogen fertilization increased (p<0.05), but there was no significant difference in dry matter yield among nitrogen fertilization levels during the heading stage (p>0.05). Chlorophyll also was significantly higher in T5 (250%) at all harvesting times, whereas nitrate nitrogen content was highest at the vegetative stage, gradually decreased as growth progressed, and lowest at the milk stage. Finally, as the nitrogen fertilization levels increased, the nitrate nitrogen content was significantly higher in all treatment groups (p<0.05). Therefore, our results suggest that the most appropriate nitrogen fertilizer levels is between 150%–200%, considering the dry matter yield, feed ingredients and nitrate nitrogen content in barnyard millet for feed.

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ABSTRACT
Ⅰ. INTRODUCTION
Ⅱ. MATERIALS AND METHODS
    1. Experimental design
    2. Investigation parameters
    3. Statistical analysis
Ⅲ. RESULTS AND DISCUSSIONS
    1. Soil and climatic conditions
    2. Barnyard millet growth characteristics
    3. SPAD values and nitrate nitrogen content
Ⅳ. CONCLUSIONS
Ⅴ. ACKNOWLEDGEMENT
Ⅵ. REFERENCES

• Bae Hun Lee(National Institute of Animal Science, RDA)
• Ki Choon Choi(National Institute of Animal Science, RDA)
• Seung Hak Yang(National Institute of Animal Science, RDA)
• Mirae Oh(National Institute of Animal Science, RDA)
• Hyung Soo Park(National Institute of Animal Science, RDA) Corresponding author