Iron deficiency is known to be a common nutritional disorder in many countries, especially among children, women of childbearing age and pregnant women. SUNACTIVE Fe-P80 is a new type of iron supplement that applies nanotechnlateology for the purpose of overcoming the disadvantages of food supplements. This study was conducted to investigate the potential adverse effects of a 28-day repeated oral dose of SUNACTIVE Fe-P80 in rats. SUNACTIVE Fe-P80 was administered once daily by gavage to Sprague-Dawley rats for 28 days at doses of 0, 500, 1,000, and 2,000 mg/kg/day. Additional recovery groups from the control and highdose groups were observed for a 14-day recovery period. At the scheduled termination, the animals were sacrificed, their organs weighed, and blood samples collected. There were no treatment-related effects in the context of clinical signs, body weight, food intake, ophthalmoscopy, urinalysis, necropsy findings, organ weights, and hematologic, serum biochemical and histopathological parameters at any dose tested. Under the present experimental conditions, the no-observed-adverse-effect level of SUNACTIVE Fe-P80 was ≥ 2,000 mg/kg/day in both the sexes, and no target organs were identified. Thus, the results suggest that SUNACTIVE Fe-P80 is relatively safe, as no treatment-related adverse effects were observed following a 28-day repeated oral dose experiment.

Abstract
INTRODUCTION
MATERIALS AND METHODS
    Experimental animals and animal husbandry
    Preparation of test substance
    Dosage selection and experimental groups
    Administration
    Clinical signs, body weight, and food consumption
    Ophthalmoscopy
    Urinalysis
    Hematology
    Serum biochemistry
    Necropsy
    Organ weights
    Histopathology
    Statistical analysis
RESULTS
    Clinical signs, body weight, and food consumption
    Urinalysis and ophthalmic examination
    Hematology
    Serum biochemistry
    Gross findings and organ weights
    Histopathology
DISCUSSION
REFERENCES

• Jeong-Hyun Lim(National Institute of Agricultural Sciences, College of Veterinary Medicine, Chonnam National University)
• Myeong-Kyu Park(Health Care Institute, Korea Testing and Research Institute)
• Heung-Sik Seo(Health Care Institute, Korea Testing and Research Institute)
• Sang-Ho Kim(Health Care Institute, Korea Testing and Research Institute)
• Woong-Il Kim(College of Veterinary Medicine, Chonnam National University)
• In-Sik Shin(College of Veterinary Medicine, Chonnam National University)
• Jong-Choon Kim(College of Veterinary Medicine, Chonnam National University) Corresponding author