Rats are one of the most widely used animals in biomedical sciences because their metabolism and physiology are comparable to humans. In recent years, gene-targeted models have been developed using various animal species utilizing engineered nucleases such as clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated gene (Cas). It has recently become possible to efficiently transfect CRISPR/Cas into embryos via electroporation. However, electroporation can damage fertilized eggs; therefore, it is important to determine the optimal embryo culture conditions. A standardized approach for routine and reproducible rat transgenesis will render rat models more accessible for research. We performed experiments to obtain rat embryos with efficient superovulation and synchronization, and to investigate the appropriate medium conditions for pronuclear stage embryos subjected to electroporation stimulation for the introduction of engineered nuclease.

Abstract
1. 서 론
2. 재료 및 방법
    2.1. 실험동물
    2.2. 발정동기화와 과배란 유도
    2.3. 배아 채란 및 배양
    2.4. 배아 전기천공
    2.5. 통계처리
3. 결과 및 고찰
    3.1. 호르몬에 의한 랫드 발정동기화와 과배란 유도효율 비교
    3.2. 배양배지의 조성이 랫드 배아의 발생에 미치는영향
    3.3. 배양환경이 전기 천공된 랫드 배아의 발생에 미치는 영향
4. 결 론
감사의 글
REFERENCES

• 이지민(서울대학교 수의과대학 실험동물의학교실) | Ji Min Lee (Department of Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea) Corresponding Author