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대두 β - amylase Isozyme 의 분리 및 정제 KCI 등재

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한국식품영양학회지 (The Korean Journal of Food And Nutrition)
한국식품영양학회 (The Korean Society of Food and Nutrition)
초록

The soybean β-amylase [α-1, 4-glucan maltohydrolase, EC. 3. 2. 1. 2] is composed of seven isozymes( Ⅰ`, Ⅰ, Ⅱ, Ⅲ, Ⅳ, Ⅴ and Ⅵ), and isozyme Ⅱ and Ⅳ are the main components among these. The purification of β-amylase isozymes from soybean whey were performed by ammonium sulfate fractionation, CM-Sephadex C-50 column chromatography, DEAE-Sephadex chromatography and Gel filtration. The resulted purity of β-amylase was throughly confirmed by electrophoresis, and then determined its isoelectric point and molecular weight. The results obtained were as follows ; 1. Five active fractions of soybean β-amylase were derived on CM-Sephadex C-50 column chromatography . 2. Seven active bands of β-amylase isozymes were detected by isoelectric focusing gel electrophoresis, and their isoelectric points ( Ⅰ` to Ⅵ) were 5.07, 5.15, 5.25, 5.40, 5.55, 5.70 and 5.93, respectively. 3. Isozyme Ⅱ and Ⅳ were main components of soybean β-amylase. 4. The molecular weights of both isozyme Ⅱ and Ⅳ were determined to be 56, 000 daltons by the result of SDS polyacrylamide gel electrophoresis. 5. Km values of main isozyme Ⅱ & Ⅳ for amylopectin were determined to be 2.25㎎/㎖, which suggest the same function of each isozyme.

저자
  • 김창렬 | Chang Ryoul Kim
  • 고대희 | Dae Hee Koh
  • 김영주 | Young Ju Kim
  • 김광현 | Kwang Hyeon Kim
  • 조인경 | In Kyeong Choi
  • 은종방 | Jong Bang Eun