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Next-Generation Sequencing을 활용한 어류 위 내용물 분석의 적용 가능성 KCI 등재

Applicability of Next-Generation Sequencing for Analysis of Stomach Contents in Fish

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  • URLhttps://db.koreascholar.com/Article/Detail/421492
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생태와 환경 (Korean Journal of Ecology and Environment)
초록

The predator-prey interaction in freshwater ecosystems is a crucial area in the ecological study field and one of example to find such interaction is to investigate stomach contents. However, traditional method through visual inspection often induce misidentification, as it depends critically on intactness of physically visible data. In this study, we utilized Next-Generations Sequencing (NGS) technology to test the applicability stomach content analysis and overcome such limitation. NGS was applied to analyze the stomach contents of the Hemibarbus labeo, Tachysurus fulvidraco, and Plecoglossus altivelis collected in the lower part of Nakdong River. As a result, T. fulvidraco had a higher number of Animalia operational taxonomic units (OTUs) intake rate than H. labeo. At the same time, P. altivelis had higher number of Plantae OTUs intake rate than T. fulvidraco and higher Protozoa OTUs intake rate than H. labeo respectively. Therefore, NGS technology application enable to overcome traditional method’s limitation and discover hidden interspecific interaction which can further be used in appropriate habitat assessment.

목차
Abstract
서 론
재료 및 방 법
    1. 조사 지점 및 조사 방법
    2. 실험 대상 어종
    3. 실험 과정
    4. 데이터 분석
결 과
고 찰
REFERENCES
저자
  • 박채진(부산대학교 생명과학과) | Chae-Jin Park (Department of Integrated Biological Science, Pusan National University)
  • 윤선빈(부산대학교 생명과학과) | Seonbin Yun (Department of Integrated Biological Science, Pusan National University)
  • 이현식(부산대학교 생명과학과) | Hyeon-Sik Lee (Department of Integrated Biological Science, Pusan National University)
  • 장서윤(부산대학교 생명과학과) | Seoyun Jang (Department of Integrated Biological Science, Pusan National University)
  • 김강희(부산대학교 생명과학과) | Kang-Hui Kim (Department of Integrated Biological Science, Pusan National University)
  • 홍동현(부산대학교 생명과학과) | Donghyun Hong (Department of Integrated Biological Science, Pusan National University)
  • 주기재(부산대학교 생명과학과) | Gea-Jae Joo (Department of Integrated Biological Science, Pusan National University) Corresponding author