This study used hydroacoustic method to identify the vertical and horizontal distribution of Antarctic silverfish in the Ross Sea, Antarctica. In February and December 2018, Antarctic silverfish was detected up to 250 meters, and was mainly distributed in water depths of 20 to 30 meters. The horizontal distribution of Antarctic silverfish was mostly undetected in February, and December showed a relatively stronger distribution than that of February. Antarctic silverfish is characterized by their distribution near sea ice.