Roridin E, a constituent of Podostroma cornu-damae, has excellent medicinal properties and exhibits powerful anticancer effects. However, being a poisonous mushroom, P. cornu-damae is difficult to find in its natural environment. Therefore, only a limited amount of roridin E is isolated from P. cornu-damae. We isolated roridin E from mycelial culture medium of P. cornu-damae and examined its effects on five breast cancer cell lines. The purity of the isolated roridin E was 96%. It exhibited anticancer effects on all five breast cancer cell lines, with an IC50 of at least 0.0006 mg/L. To the best of our knowledge, this is the first report on the investigation of effects of roridin E isolated from the mycelium of P. cornu-damae mushrooms on five breast cancer cell lines. However, safety issues should be investigated and addressed in future studies before roridin E is used in clinical settings.

INTRODUCTION
MATERIALS AND METHODS
    Microorganism
    Submerged culture
    Extract and isolation of roridin E
    Analytical TLC and UV-Visible spectroscopy
    High pressure liquid chromatography analysis
    Antioxidant properties
    Cell lines and culture
    The MTT cell viability assay for cytotoxicity testing
    Scratch wound healing assay
RESULTS AND DISCUSSION
    Isolation of roridin E from P. cornu-damae culturebroth
    Characterization of the isolated compound used UVVisiblespectroscopy
    HPLC spectrum of isolated roridin E
    Antioxidant activity of isolated roridin E
    Anticancer activity of isolated roridin E
    Effect of wound-healing in vitro
CONCLUSION
ACKNOWLEDGEMENTS
Data Availability
Conflict of Interest
Authors' Contributions

• Si Young Ha(Department of Environmental Materials Science/Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Republic of Korea)
• Dong Hwan Lee(Forest Medicinal Resources Research Center, National Institute of Forest Science, Youngju 36040, Republic of Korea)
• Ji Young Jung(Department of Environmental Materials Science/Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Republic of Korea)
• Han Min Park(Department of Environmental Materials Science/Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Republic of Korea)
• Jae-Kyung Yang(Department of Environmental Materials Science/Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Republic of Korea) Corresponding author