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pp.347-353
토양에서 분리한 Streptomyces lydicus YSK-681이 생산하는 메치실린내성 황색 포도상구균에 유효한 항생물질을 정제하여 물리화학적 특성을 조사하였다. 물질정제는 chloroform으로 추출한 후 silica gel column chromatography, Si, ODS, GPC HPLC순으로 진행하였다. FAB MS의 분자량 측정결과 m/z 1022.4와 1036.4(M+H)^+의 두 개의 피크가 분석되었으며, NMR 분석에서는 -OH와 leucine으로 추정되는 관능기가 조사되었다. 정제된 항생물질의 MIC는 그람 양성균에 대해서 1∼32 ug/ml그람음성균과 효모에서는 125 ug/ml 이상이었다. 임상에서 분리된 129개의 MRSA에 대한 MIC는 8 ug/ml이었으며, 암세포인 P388, HeLa, S180에 대한 세포독성은 없었다.
An antibiotic for methicillin resistant Strephylococcus aureus(MRSA) produced by Streptomyces lydicus YSK-681 was extracted by chloroform, and then purified by the C_18 reversed-phase HPLC and silica gel column HPLC. The molecular weight of the purified antibiotic were determined from the FAB analysis MS as m/z 1022.4 and 1036.4(M+H)^+, indication that the iosolated antibiotic consisted of two similar compounds with the molecular weight difference of 14 m/z value. With the aid of the various nuclear magnetic resonance(NMR) spectroscopic techniques such as ^1H NMR, ^13C NMR, DEPT and HMQC spectroscopy, the characteristice of functional groups were deduced as the hydroxyl group and leucine. The MIC values of the purified antibiotic were observed at 1∼32 ug/ml against Gram-positive bacteria compared to > 125 ug/ml against Gram-negative bacteria or fungi. The anibiotic was active at 8 ug/ml of MIC_90 against 129 clinical isolates of MRSA. The antibiotic showed no cytotoxicity against P388 HeLa, and S180 at the concentration of 500 ug/ml.
