논문 상세보기
Chlorothalonil is continuously introduced into the marine environment and has significant toxic effects on various marine organisms, however, research on its effect on seaweed is limited. Therefore, we analyzed the impact of chlorothalonil on the early life stages of major aquaculture species in Korea, Undaria pinnatifida. U. pinnatifida female gametophytes were exposed to different concentrations of chlorothalonil (0, 0.03, 0.05, 0.10, 0.20, and 0.40 mg L-1), and their survival rate and relative growth rate were analyzed. The no observed effect concentration (NOEC), lowest observed effect concentration (LOEC), and median lethal concentration (LC50) for female gametophyte survival were determined as 0.05, 0.10, and 0.141 (0.121-0.166) mg L-1. NOEC, LOEC, and median effective concentration (EC50) for relative growth rate were 0.10, 0.20, and 0.124 (0.119-0.131) mg L-1. Therefore, female gametophytes of U. pinnatifida are expected to experience toxic effects at concentrations above 0.05-0.10 mg L-1 of chlorothalonil. These research findings are anticipated to serve as crucial reference data for evaluating the effects of chlorothalonil on the health of U. pinnatifida in the early life stages.
2. 재료 및 방법
2.1. 시험생물
2.2. Chlorothalonil 농도 조성
2.3. 배양조건
2.4. 암배우체 생존율
2.5. 암배우체 상대성장률
2.6. 통계분석
3. 결 과
3.1. 암배우체의 생존율
3.2. 암배우체 상대성장률
3.3. U. pinntifida 암배우체를 이용한 CTN 영향평가
4. 고 찰
적 요
사 사
