Tulsi (Ocimum sanctum), commonly known as Holy Basil is a revered herb with a rich history in traditional medicine systems, particularly in Southeast Asia. For its medicinal properties, Tulsi has been regarded as an “Elixir of Life” and has been used to treat various ailments. However, the comprehensive investigation of Tulsi extracts and their potential pharmacological benefits, specifically in relation to antioxidant activity remains limited. Hence, the objective of this study was to evaluate the antioxidant activity of Tulsi leaf and stem extract using various screening methods. We investigate the antioxidant activity exhibited by the extract using three different methods involved the utilization of the total polyphenol content assay, the ferric reducing power assay and 2, 2-diphenyl-1-14 picrylhydrazyl (DPPH) assays. The results revealed that the Tulsi leaf extract (TLE) exhibited significantly higher antioxidant activity when compared to the Tulsi stem extract (TSE) in all the performed assays. The higher content of phenolics in TLE may have contributed to its superior antioxidant activity. The HPLC (high performance liquid chromatography) analysis of TLE revealed the presence of eugenol, active compound for several therapeutic properties. These findings provide an understanding of the bioactive compounds present in Tulsi extracts and their potential antioxidant benefits.

INTRODUCTION
MATERIALS AND METHODS
    Preparation of Tulsi leaf extract (TLE) and Tulsi stemextract (TSE)
    Measurement of total polyphenol content
    DPPH radical scavenging assay
    Reducing power assay
    Quantification of eugenol content in TLE using HPLCanalysis
    Statistical analysis
RESULTS
    Determination of total Polyphenol contents of TLEand TSE
    Determination of radical scavenging activities of TLEand TSE
    Determination of total ferric reducing ability of TLEand TSE
    Determination of eugenol content in TLE using HPLCanalysis
DISCUSSION

• Most Nusrat Zahan(College of Veterinary Medicine, Gyeongsang National University, Jinju, 52828, Korea)
• Jong Hyuk Kim(Institutes of Agriculture and Life Science, Gyeongsang National University, Jinju, 52828 Korea)
• Hyeryeon Yang(College of Veterinary Medicine, Gyeongsang National University, Jinju, 52828, Korea)
• Sin Yeong Park(College of Veterinary Medicine, Gyeongsang National University, Jinju, 52828, Korea)
• Min Yeong Kim(College of Veterinary Medicine, Gyeongsang National University, Jinju, 52828, Korea)
• Il Rae Rho(Institutes of Agriculture and Life Science, Gyeongsang National University, Jinju, 52828 Korea)
• Euikyung Kim(College of Veterinary Medicine, Gyeongsang National University, Jinju, 52828, Korea, Institutes of Animal Medicine Gyeongsang National University, Jinju 52828, Korea)
• Changkeun Kang(College of Veterinary Medicine, Gyeongsang National University, Jinju, 52828, Korea, Institutes of Animal Medicine Gyeongsang National University, Jinju 52828, Korea) Corresponding Author