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수생태계 건강성 조사ㆍ평가를 위한 실내분석 정도관리 방법: 부착돌말류 영구표본 분석도구 개발 KCI 등재

The Quality Control Method in the Laboratory Analysis of Aquatic Ecosystem Health Monitoring and Assessment: Permanent Mounting Slides Tool Development Using Benthic Attached Diatoms

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  • URLhttps://db.koreascholar.com/Article/Detail/427719
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생태와 환경 (Korean Journal of Ecology and Environment)
초록

Benthic attached diatoms (BADs), a major primary producer in lotic stream and river ecosystems are micro-sized organisms and require a highly magnified microscopic technique in the observation work. Thus, it is often not easy to ensure accuracy and precision in both qualitative and quantitative analyses. This study proposed a new technique applicable to improve quality control of aquatic ecosystem monitoring and assessment using BADs. In order to meet the purpose of quality control, we developed a permanent mounting slide technique which can be used for both qualitative and quantitative analyses simultaneously. We designed specimens with the combination of grid on both cover and slide glasses and compared their efficiency. As a result of observation and counting of BADs, the slide glass designed with the color-lined grid showed the highest efficiency compared to other test conditions. We expect that the method developed in this study could be effectively used to analyze BADs and contributed to improve the quality control in aquatic ecosystem health monitoring and assessment.

목차
서 론
재료 및 방 법
    1. 부착돌말류 실내분석 정도관리용 영구표본 슬라이드제작 준비
    2. 부착돌말류 실내분석 정도관리용 영구표본 슬라이드개발 및 비교
결과 및 고 찰
    1. 부착돌말류 실내분석 정도관리용 영구표본 슬라이드분석도구의 특성 비교
    2. 부착돌말류 실내분석 정도관리용 슬라이드 분석도구개발의 기대효과 및 제언
적 요
저자
  • 신재기(수생태원 한강(韓江)) | Jae-Ki Shin (Limnoecological Science Research Institute Korea (THE HANGANG))
  • 김난영(건국대학교 환경보건과학과) | Nan-Young Kim (Department of Environmental Health and Science, Konkuk University, Seoul 05029, Republic of Korea)
  • 박용은(건국대학교 사회환경공학부) | Yongeun Park (School of Civil and Environmental Engineering, Konkuk University, Seoul 05029, Republic of Korea)
  • 이경락(국립환경과학원 물환경연구부) | Kyung-Lak Lee (Water Environmental Research Department, National Institute of Environmental Research, Incheon 22689, Republic of Korea)
  • 김백호(한양대학교 생명과학과) | Baik-Ho Kim (Department of Life Science, Hanyang University, Seoul 04763, Republic of Korea)
  • 김용재(대진대학교 생명과학과) | Yong-Jae Kim (Department of Life Science, Daejin University, Gyeonggi 11159, Republic of Korea)
  • 김한순(경북대학교 생물학과) | Han-Soon Kim (Department of Biology, Kyungpook National University, Daegu 41566, Republic of Korea)
  • 이정호(대구대학교 생물교육과) | Jung Ho Lee (Department of Biology Education, Daegu University, Gyeongbuk 38453, Republic of Korea)
  • 이학영(전남대학교 생물학과) | Hak Young Lee (Department of Biology, Chonnam National University, Gwangju 61186, Republic of Korea)
  • 황순진(수생태원 한강(韓江)) | Soon-Jin Hwang (Limnoecological Science Research Institute Korea (THE HANGANG)) Corresponding author