We aimed to develop outstanding domestic varieties suitable for both columnar and cylindrical-shape substrates, aiming to replace foreign varieties. and bred a high-quality new strain named ‘Charmjon’, using genetic resources collected from Japan and China. The optimal cultivation temperature for Charmjon’s mycelial growth was found to be 25°C, and its mycelial growth at 15°C and 25°C was superior to the control variety. In terms of mycelial growth characteristics based on the substrate, Charmjon exhibited excellent mycelial strength on PDA medium compared to the control variety. Through columnar and cylindrical-shape substrates cultivation, we assessed the quantity and morphological characteristics of the fruiting bodies. The results confirmed that Charmjon can be produced stably using both cultivation methods, and it showed higher yields and individual weights than the control variety. In addition, the color of the pileus was notably darker, and the shape of the pileus varied depending on the cultivation method. The test of genetic diversity revealed that Charmjon has distinct genetic characteristics compared to the control varieties.

• 임지훈(농촌진흥청 국립원예특작과학원 인삼특작부 버섯과) | Ji-Hoon Im (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA, Chungbuk Eumseong 27709, Korea) Corresponding author
• 오연이(농촌진흥청 국립원예특작과학원 인삼특작부 버섯과) | Youn-Lee Oh (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA, Chungbuk Eumseong 27709, Korea)
• 오민지(농촌진흥청 국립원예특작과학원 인삼특작부 버섯과) | Minji Oh (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA, Chungbuk Eumseong 27709, Korea)
• 김민식(농촌진흥청 국립원예특작과학원 인삼특작부 버섯과) | Minseek Kim (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA, Chungbuk Eumseong 27709, Korea)
• 장갑열(농촌진흥청 국립원예특작과학원 인삼특작부 버섯과) | Kab-Yeul Jang (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA, Chungbuk Eumseong 27709, Korea)