This study was performed to investigate antioxidant and anti-inflammatory activities of perilla(Perilla frutescens L.) seed, flower and leaf according to extraction condition. Perilla seed extracts(PSE), perilla flower extracts(PFE), perilla leaf extracts(PLE) was extracted by stirring extraction (STE, 25°C), shaking extraction (SHE, 80°C), and sonication assisted extraction(SAE, , 25°C) with 94% ethanol, 60% ethanol and distilled water, followed by analysis of total polyphenol and flavonoid and testing radical scavenging activities. The highest total polyphenol content (5.47, 9.36, 38.58 mg gallic acid equivalent/g), total flavonoid content(5.77, 8.62, 46.44 mg catechin equivalent/g), ABTS(10.68, 19.46, 63.56 mg trolox equivalent/g) and DPPH(6.51, 7.69, 79.73 mg trolox equivalent/g) radical scavenging activity of PSE, PFE and PLE was observed in the HWE with 60% ethanol,. Among the three extraction method, SHE provided the best results for yield, polyphenol, flavonoid content of perilla seed, flower, leaf in comparison to STE or SAE. SHE with 60% ethanol of perilla seed, flower, leaf more effectively inhibited secretion of nitric oxide(NO) and pro-inflammatory cytokine in RAW 264.7 macrophage exposed to LPS compared to other extraction solvent and method. Therefore, these extracts obtained from perilla seed, flower, leaf could be used antioxidant and anti-inflammatory ingredients in the food industry.

서 론
재료 및 방법
    1. 실험재료 및 추출물 제조
    2. 총 폴리페놀 함량 측정
    3. 총 플라보노이드 함량 측정
    4. ABTS 라디칼 소거능 측정
    5. DPPH 라디칼 소거능 측정
    6. 세포배양 및 독성 측정
    7. 산화질소(nitric oxide) 및 염증성 사이토카인(cytokine)분석
    8. 통계분석
결과 및 고찰
    1. 총 폴리페놀 및 플라보노이드 함량
    2. 항산화 활성
    3. 항염증 활성
    4. 상관관계분석
요약 및 결론
감사의 글
References

• 김민영(농촌진흥청 국립식량과학원 남부작물부 밭작물개발과 농업연구사) | Min Young Kim (Associate Researcher, Upland Crop Breeding Research Division, Dept. of Sourthern Area Crop Science, National Institute of Crop Science, RDA, Miryang 50424, Korea) Corresponding author
• 김정인(농촌진흥청 국립식량과학원 남부작물부 밭작물개발과 농업연구사) | Jung In Kim (Associate Researcher, Upland Crop Breeding Research Division, Dept. of Sourthern Area Crop Science, National Institute of Crop Science, RDA, Miryang 50424, Korea)
• 김상우(농촌진흥청 국립식량과학원 남부작물부 밭작물개발과 농업연구사) | Sang Woo Kim (Associate Researcher, Upland Crop Breeding Research Division, Dept. of Sourthern Area Crop Science, National Institute of Crop Science, RDA, Miryang 50424, Korea)
• 김성업(농촌진흥청 국립식량과학원 남부작물부 밭작물개발과 농업연구사) | Sungup Kim (Associate Researcher, Upland Crop Breeding Research Division, Dept. of Sourthern Area Crop Science, National Institute of Crop Science, RDA, Miryang 50424, Korea)
• 오은영(농촌진흥청 국립식량과학원 남부작물부 밭작물개발과 농업연구사) | Eunyoung Oh (Associate Researcher, Upland Crop Breeding Research Division, Dept. of Sourthern Area Crop Science, National Institute of Crop Science, RDA, Miryang 50424, Korea)
• 이정은(농촌진흥청 국립식량과학원 남부작물부 밭작물개발과 농업연구사) | Jeongeun Lee (Associate Researcher, Upland Crop Breeding Research Division, Dept. of Sourthern Area Crop Science, National Institute of Crop Science, RDA, Miryang 50424, Korea)
• 이은수(농촌진흥청 국립식량과학원 남부작물부 밭작물개발과 농업연구사) | Eunsoo Lee (Associate Researcher, Upland Crop Breeding Research Division, Dept. of Sourthern Area Crop Science, National Institute of Crop Science, RDA, Miryang 50424, Korea)
• 안연주(농촌진흥청 국립식량과학원 남부작물부 밭작물개발과 산학협동과정 박사과정생) | Yeon Ju An (Doctor's Student, Upland Crop Breeding Research Division, Dept. of Sourthern Area Crop Science, National Institute of Crop Science, RDA, Miryang 50424, Korea)
• 이명희(농촌진흥청 국립식량과학원 남부작물부 밭작물개발과 농업연구관) | Myoung Hee Lee (Research Officer, Upland Crop Breeding Research Division, Dept. of Sourthern Area Crop Science, National Institute of Crop Science, RDA, Miryang 50424, Korea)
• 김춘송(농촌진흥청 국립식량과학원 남부작물부 밭작물개발과 농업연구관) | Choon-Song Kim (Research Officer, Upland Crop Breeding Research Division, Dept. of Sourthern Area Crop Science, National Institute of Crop Science, RDA, Miryang 50424, Korea)