GC-MS/MS using liquid-liquid extraction (LLE) and C18 cartridges was used to identify and quantify levels of chlorpyrifos, chlorpyrifos-methyl, cypermethrin, deltamethrin and permethrin in bulk raw milk. A calibration curve spanning 10 ng/mL to 200 ng/mL was obtained with a satisfactory correlation coefficient of 0.99. The limits of detection (LOD) and limits of quantitation (LOQ) for chlorpyrifos, chlorpyrifos-methyl, cypermethrin, deltamethrin, and permethrin in the matrix ranged from 0.06 to 1.81 ng/mL and 0.19 to 6.04 ng/mL, respectively. The recoveries of 5 pesticides from spiked samples at 37.5-125 ng/mL ranged from 86.1 to 102.1%. The measurement of uncertainty of the GC-MS/MS method for these five pesticides was developed based on the analytical process and quantification. An analysis method that is easier and faster than the method specified in the Korean food standards codes for analyzing these five pesticides in raw material milk was developed. Moreover, the analytical method for chlorpyrifos, chlorpyrifos-methyl, cypermethrin, deltamethrin, and permethrin in bulk raw milk by GC-MS/MS was established.

Materials and Methods
    시약 및 재료
    시료 전처리 방법
    기기분석 조건
    Method validation
Results and Discussion
    시료전처리 및 분석법 확립
    선택성 및 특이성
    직선성
    일내, 일간 정밀성 및 정확성
    회수율, 검출한계(LOD) 및 정량한계(LOQ)
    측정불확도 결과
Conclusion
Acknowledgement
국문요약
ORCID

• 최영내(한국식품과학연구원) | Young Nae Choi (Korea Advanced Food Research Institute, Uiwang, Korea)
• 신윤호(유한대학교 건강생명학부 유한생명바이오학과) | Yoon ho Shin (Dept. of Yuhan Biotechnology, Schcool of Bio-Health Sciences, Yuhan University Bucheon, Korea)
• 조황의(전북대학교 약학대학) | Hwangeui Cho (School of Pharmacy, Jeonbuk National University, Jeonju-si, Korea) Corresponding author
• 김정복(유한대학교 건강생명학부 유한생명바이오학과) | Jung Bok Kim (Dept. of Yuhan Biotechnology, Schcool of Bio-Health Sciences, Yuhan University Bucheon, Korea) Corresponding author