Aquatic ecosystem is composed of various organic and inorganic matters. Its health is essential for sustaining its members such as vegetations, mammals, birds, fishes and invertebrates. Samcheonpo stream is one of candidate streams in Sacheon area of ecological restoration. For this reason, this study conducted to build up a data base for future comparison. The results shows that biodiversity of Epilithic Diatoms and Invertebrates is below average which means that human impacts such as artificial structures and activities have been negatively related to stream health. Consequently, we suggest several key factors when ecological restoration is performed in the stream.