In this study, the impact load resulting from collision with the fuel rods of surrogate spent nuclear fuel (SNF) assemblies was measured during a rolling test based on an analysis of the data from surrogate SNF-loaded sea transportation tests. Unfortunately, during the sea transportation tests, excessive rolling motion occurred on the ship during the test, causing the assemblies to slip and collide with the canister. Hence, we designed and conducted a separate test to simulate rolling in sea transportation to determine whether such impact loads can occur under normal conditions of SNF transport, with the test conditions for the fuel assembly to slide within the basket experimentally determined. Rolling tests were conducted while varying the rolling angle and frequency to determine the angles and frequencies at which the assemblies experienced slippage. The test results show that slippage of SNF assemblies can occur at angles of approximately 14° or greater because of rolling motion, which can generate impact loads. However, this result exceeds the conditions under which a vessel can depart for coastal navigation, thus deviating from the normal conditions required for SNF transport. Consequently, it is not necessary to consider such loads when evaluating the integrity of SNFs under normal transportation conditions.