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수온과 염분이 참전복, Haliotis discuss hannai 의 혈림프액 성상과 유리아미노산 조성에 미치는 영향 KCI 등재
Effects of Temperature and Salinity on the Hemolymp Characteristic and Composition of Free Amino Acid of the Abalone, Haliotis discuss hannai
pp.50-55
본 연구결과 glucose, NH3, AST, ALT는 4℃일 때 가장 높게 나타났으며, glucose와AST는 4℃일 때 다른 염분구보다 26 psu에서 유의하게 높았다. 참전복에는 총 18가지 FAA가 존재하였으며, taurine, arginine, glutamic acid, alanine, glycine, lysine이 전체의 90% 가량을 차지하는 우점 FAA 로 나타났다. Taurine은 30 psu 이상에서는 수온 간 유의한 차이가 없었으나, 26 psu에서는 6, 8℃일 때 유의하게 증가하였다. Glycine은 수온이 낮아질수록 유의하게 감소하여 6℃일 때 가 장 높게 나타났다. 따라서 참전복은 30~34 psu, 8~10℃에서의 조건으로 수송 시 스트레스를 최소화할 수 있을 것으로 여겨진다.
Glucose was continuously increased as time was elapsed under the temperature at 6℃ and 4℃. NH3 was significantly increased at 4℃. AST was increased as temperature and salinity were decreased which indicated that it was affected by temperature, salinity and interaction of temperature×salinity. ALT was significantly increased as temperature was decreased. Free amino acid contents, excluding aspartic acid, threonine, serine and glycine were increased as temperature was decreased. This decreasing tendency was also observed when salinity was decreased. Taurine, aspartic acid, glutamic acid and arginine were significantly increased as salinity was decreased, while glycine, alanine, methionine, tyrosine, and histidine were decreased as salinity was decreased. The result of this study suggested that transport condition to minimize the stress is considered in the range of 30~34 psu, 8~10℃.
재료 및 방법
1. 실험생물 및 실험조건
2. 혈림프액 채취
3. 혈림프액 성분분석
4. 유리아미노산(Free amino acid, FAA) 분석
5. 통계처리
결 과
1. 혈림프액 parameters 변화
2. 조직 내 FAA 함량 변화
고 찰
