Heat shock proteins (HSPs) are highly conserved cellular proteins that contribute to adaptive responses of organisms to a variety of stressors. In response to stressors, cellular levels of HSPs are increased and play critical roles in protein stability, folding and molecular trafficking. The mRNA expression pattern of two well-known heat shock protein transcripts, HSP70 and HSP90 were studied in two tissues of nerve ganglia, cerebral ganglion and pleuropedal ganglion of Pacific abalone (Haliotis discus hannai). It was observed that both HSP70 and HSP90 transcripts were upregulated under heat stress in both ganglion tissues. Expression level of HSP70 was found higher than HSP90 in both ganglia whereas cerebral ganglion showed higher expression than pleuropedal ganglion. The HSP70 and HSP90 showed higher expression at Day-1 after exposed to heat stress, later decreased at Day-3 and Day-7 onwards. The present result suggested that HSP70 and HSP90 synthesize in nerve ganglion tissues and may provide efficient protection from stress.

Introduction
Materials and Methods
    1. Abalone collection and rearing
    2. Experimental design
    3. Abalone husbandry and monitoring
    4. Ethics statement
    5. Sample collection
    6. Total RNA extraction and cDNA synthesis
    7. Quantitative real time PCR (qRT-PCR) analysisof abalone heat shock protein gene transcript, HSP-70 and HSP90
    8. Statistical analysis
Results and Discussions

• Zahid Parvez Sukhan(Department of Fisheries Sciences, Faculty of Aquatic Biology, Chonnam National University, Jeonnam 59626, Korea)
• Kang Hee Kho(Department of Fisheries Sciences, Faculty of Aquatic Biology, Chonnam National University, Jeonnam 59626, Korea) Corresponding author