Chromosomal level genome draft was assembled using a Korean Diadegma fenestrale (Jeju strain, KJ09). This assembly was achieved through a combined approach utilizing Nanopore long-read sequencing (approximately 134X coverage) and Illumina NovaSeq short-read sequencing (approximately 83X coverage). The assembled genome spans 243 Mb, comprises 160 scaffolds, with most of the genome contained within 11 chromosomal level scaffolds. The completeness of the assembly is reflected in BUSCO assessment, with values reaching 99.6%. Gene annotation is not completed. A symbiotic virus, Diadegma fenestrale Ichnovirus (DfIV) genome revealed 62 non-overlapping circular DNA segments the aggregate genome size was approximately 240 kb. Although the frequency of DfIV genome integration into the host’s 11 chromosomes varies from 0 to 32%, it was confirmed that all 62 DfIV genome fragments were inserted into the host genome. A total of 123 ORFs were predicted from the DfIV genome and most of those were expressed in the host’s ovary. This result may be contradictory to existing theories, but we propose a new hypothesis that some genes possessed by viruses may play different roles depending on the type and state of the host. Additionally, this phenomenon can be considered in relation to coevolution with the hosts.

• Juil Kim(Department of Plant Medicine, College of Agriculture and Life Science, Kangwon National University) Corresponding author