Ramulus mikado (Rehn, 1904) is an environmentally problematic species that has recently caused a severe outbreak on the mountainsides of the Seoul metropolitan area, resulting in significant damage to forests and causing inconvenience to people in the city centre vicinity. In this investigation, we analysed the genetic diversity through sampling in the outbreak region. The outcomes confirmed the efficiency of COI for species identification and the usefulness of haplotype analysis in examining regional variation.