Bacillus thuringiensis (Bt) is currently the most commonly used microbial pesticide. In the previous study, Bt IMBL-B9 known for its high toxicity against Spodoptera exigua, S. frugiperda and Plutella xylostella was characterized. To develop novel biopesticide, optimization of culture medium is required for the cost-effective mass production for toxin production of IMBL-B9. Through experimental design by Plackett-Burman design, ingredients that significantly influenced the production of IMBL-B9's toxin were selected. Using these results, the novel culture medium for IMBL-B9 was developed and the toxin yield of IMBL-B9 was significantly increased than conventional media by using this medium. These results could be useful for the development of biopesticides.

• Siyi Liu(Department of Agricultural Biotechnology, Seoul National University) Corresponding author
• Dong Hwan Park(Research Institute for Agriculture and Life Sciences, Seoul National University)
• Jae Young Choi(Research Institute for Agriculture and Life Sciences, Seoul National University)
• Minghui Wang(Department of Agricultural Biotechnology, Seoul National University)
• Yeon Ho Je(Department of Agricultural Biotechnology, Seoul National University, Research Institute for Agriculture and Life Sciences, Seoul National University)