Parasitic rate of A. orientalis was calculated and compared with parasitic rate five years ago, immediately after release, and parasitic rate was also compared according to the distance away from the release point. In addition, haplotype of A. orientalis was confirmed and the association with life cycle characteristics was decided. The survey was conducted in 2022 and 2023. The parasitic rate of release point in Buyeo increased significantly from 3.6% in 18 years to 91.21% in 22. And the parasitic rate by distance from release point is 91.21% (200m), 44.46% (12km), and 65.75% (46km). At all points of survey, only haplotype C and D were found, and there was no significant difference between haplotype C and D in the parasitic rate and sex ratio.

• Yeongmo Kim(Animal Systematics Lab., Department of Biological Science, Kunsan National University) Corresponding author
• Yeonghyeok Yu(Animal Systematics Lab., Department of Biological Science, Kunsan National University)
• Duk-Young Park(Insect Biosystematics Laboratory, Department of Agricultural Biotechnology, Seoul National University)
• Seunghwan Lee(Insect Biosystematics Laboratory, Department of Agricultural Biotechnology, Seoul National University, Research Institute of Agriculture and Life Sciences, Seoul National University)
• Hyojoong Kim(Animal Systematics Lab., Department of Biological Science, Kunsan National University)