Although ethylformate and phosphine fumigants are widely used for pest quarantine, studies related to their mechanism of action and metabolic physiological changes in Drosophila models are still unclear. In this study, we investigated how key metabolites altered by fumigants and cold treatment are associated with and affect insect physiology by comparative metabolome analysis. Fumigant treatment significantly altered cytochrome P450 and glutathione metabolites involved in the detoxification mechanism and showed lower expression of PGF2α involved in the immune response compared to the control. Additionally, most of the metabolites functioned in metabolic pathways related to the biosynthesis of amino acids, nucleotides and cofactors.

• Hyun-Na Koo(Department of Plant Medicine, Chungbuk National University) Corresponding author
• Ha Hyeon Moon(Department of Plant Medicine, Chungbuk National University)
• Yuno Lee(Department of Plant Medicine, Chungbuk National University)
• Dong Hyun Kang(Department of Plant Medicine, Chungbuk National University)
• Hyunkyung Kim(Department of Plant Medicine, Chungbuk National University)
• Junbeom Lee(Metabolomics Research Center for Functional Materials, Kyungsung University)
• Gil-Hah Kim(Department of Plant Medicine, Chungbuk National University)