We analyzed trend of plants (40 items) on imported seedlings using the Pest Information System (PIS) for the past 20 years. 530 species (9 order 336 genus) were found among 40 items on imported seedlings including Rosaceae, Arecaceae, Crassulaceae, and Fabaceae. Hemiptera were the most expected to be detected pest as 52%, followed by Coleoptera as 16%, Lepidoptera as 11%, and Diptera as 8% in potential detection rate. For prohibited pests, Tephritidae are the most common pests as 86% in approximately 60 prohibited species, including Bactrocera dorsalis, and Bactrocera tryoni in Diptera and Cydia pomonella in Lepidoptera.