The optimization of a prevention program utilizing a novel trivalent inactivated Salmonella bacterial vaccine to protect poultry from Salmonella infections was evaluated in this study. A total of 50 brown nick layers were divided into 5 groups, A to E, each containing 10 hens. Group B hens were immunized with the SG9R vaccine, group C chickens were immunized with the trivalent inactivated Salmonella bacterial vaccine, and group D hens were primed with SG9R and boosted with the trivalent inactivated Salmonella bacterial vaccine. Group E hens were injected with sterile PBS. All hens in groups B to E were orally challenged with a mixture of wild-type S. Enteritidis, S. Typhimurium, and S. Gallinarum (approximately 6×10⁹ CFU/ 0.2 ml/bird). Serum IgG titers, CD3+CD4+ T-cell levels, and CD3+CD8+ T-cell levels in group D were significantly higher than those in group A. Additionally, all animals in groups A to D showed no clinical symptoms and survived after the virulent challenges, whereas all chickens in group E died following the challenge. The challenge strains of S. Enteritidis, S. Typhimurium, and S. Gallinarum were not isolated from the liver, spleen, cecum, and cloaca of group D chickens. These findings indicate that priming with SG9R and boosting with a trivalent inactivated Salmonella bacterial vaccine can be an effective approach for preventing Salmonella infections by inducing robust protective humoral and cellular immune responses in chickens.

서 론
재료 및 방법
    실험에 사용된 균주, 배양조건 및 프라이머
    실험 동물
    3가 살모넬라 불활화 사균체 백신 제조
    백신 접종 및 가검물 채취
    체액성 면역 반응 측정
    세포성 면역반응 측정
    공격접종 및 생존율과 각 조직에서의 공격접종 균주 분리
    통계분석
결 과
    각 살모넬라 OMPs에 대한 IgG 항체 역가
    CD3+CD4+ T-cells, CD3+CD8+ T-cells
    공격접종 후 생존율 및 부검 결과
    균분리 결과
고 찰
감사의 글
REFERENCES

• Yeong Ju Yu(Department of Veterinary Public Health, College of Veterinary Medicine, Jeonbuk National University, Iksan 54596, Korea)
• Jeong Hee Yu(Department of Veterinary Public Health, College of Veterinary Medicine, Jeonbuk National University, Iksan 54596, Korea)
• Jin Hur(Department of Veterinary Public Health, College of Veterinary Medicine, Jeonbuk National University, Iksan 54596, Korea) Corresponding author