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Feeding, excretion, survival, and histological alterations in zebrafish Danio rerio from single and combined exposure to microplastics and copper KCI 등재
- 언어ENG
- URLhttps://db.koreascholar.com/Article/Detail/432627
This study evaluated the risk of single and combined exposure to microplastics in zebrafish (Danio rerio) through biomarkers, such as survival rate, excretion rate, and histological alterations of organ systems. The experimental groups were the control (Cont.), single microplastics exposure group (MPs, 1.83%/fish total weight (g)), the copper group (Cu, 21.6 μg L-1), and a group with combined exposure to MPs and copper (MPs*Cu). The experiment was conducted with individual exposure (7 days) for MP excretion rate analysis and group exposure (14 days) for biomarker analysis. The daily excretion rate of MPs tended to decrease in a time-dependent manner. The copper concentration in the body was not significantly different between single and combined copper exposure. The degeneration of mucous cells in the skin, capillary dilation of the gill lamella, increased intestinal mucous, hepatocyte hypertrophy, and the degeneration of glomeruli and renal tubules were observed in all exposure groups. These histological alterations showed the highest tendency in the MPs*Cu group. In this study, the changes in biomarkers were attributed to the single effect of copper or the combined effect of copper and MPs rather than being solely influenced by MPs.
2. MATERIALS AND METHODS
2.1. Exposure materials
2.2. Ethics statement
2.3. Experimental fish
2.4. Experimental design
2.5. Analysis
3. RESULTS
3.1. Excretion rate of MPs
3.2. Copper concentrations in the body tissue
3.3. Survival rate
3.4. Histological changes
4. DISCUSSION
CRediT authorship contribution statement
Declaration of Competing Interest
ACKNOWLEDGEMENTS
REFERENCES
