Tricholoma matsutake is a traditional favorite food in East Asia, cultivated in fairy rings called “shiro,” which are found near Pinus densiflora. For effective artificial cultivation of Tri. matsutake, microorganisms from symbiotic fairy rings are co-cultivated. In this study, one bacterial isolate (Y22_B35) and two fungal isolates (Y22_F64 and Y22_F68) displayed growth-promoting effects on Tri. matsutake mycelium (158.47, 125.00, and 122.26% enhanced growth, respectively). For identification, 16S rRNA or ITS regions from the microorganisms¡¯ genomes were sequenced. Other sequences, including BenA, CaM, and RPB2 were sequenced in the fungal isolates. The bacterial isolate Y22_B35 was identified as Bacillus cereus. Y22_F64 and Y22_F68 were identified as Umbelopsis nana and Aspergillus parvulus, respectively. To identify the effects of the dominant microorganisms on Tri. Matsutake cultivation, metagenomic analyses were performed. Discovery of these Tri. matsutake mycelium growth-promoting microorganisms and metagenomics analyses are expected to contribute to our understanding of Tri. matsutake fruiting body growth and construction of biomimicry.

• 최두호(농진청 국립원예특작과학원 인삼특작부 버섯과) | Doo-Ho Choi (Mushroom Research Division, National Institute of Horticultural and Herbal Science, RDA, Eumseong, Chungbuk 27709)
• 이은지(농진청 국립원예특작과학원 인삼특작부 버섯과) | Eunji Lee (Mushroom Research Division, National Institute of Horticultural and Herbal Science, RDA, Eumseong, Chungbuk 27709)
• 이강효(농진청 국립원예특작과학원 인삼특작부 버섯과) | Kang-Hyo Lee (Mushroom Research Division, National Institute of Horticultural and Herbal Science, RDA, Eumseong, Chungbuk 27709)
• 안기홍(농진청 국립원예특작과학원 인삼특작부 버섯과) | Gi-Hong An (Mushroom Research Division, National Institute of Horticultural and Herbal Science, RDA, Eumseong, Chungbuk 27709) Corresponding author