This study investigated major constituents and anti-inflammatory effects of an ethanol extract of Platycodon grandiflorum leaves. Through HPLC analysis, chlorogenic acid and luteolin-7-O-glucoside were identified as predominant constituents in the ethanol extract. Their anti-inflammatory effects were evaluated using murine macrophage (RAW 264.7 cells) and human lung carcinoma cells (NCI-H292 & A549). The ethanol extract significantly (p<0.01) inhibited the production of nitrite, interleukin-6 (IL-6), and prostaglandin E2 (PGE2) induced by lipopolysaccharide (LPS) in RAW 264.7 cells. Furthermore, the ethanol extract suppressed the expression of cyclooxygenase-2 (COX-2) and inducible NO synthase (iNOS) proteins in RAW 264.7 cells stimulated with LPS. In NCI-H292 and A549 cells, treatment with the ethanol extract significantly (p<0.05) decreased levels of pro-inflammatory cytokines IL-6 and IL-8 induced by IL-1β. The phosphorylation of ERK rather than JNK in the mitogen-activated protein kinase signaling pathway was observed to be a more important mediator in the down-regulation of pro-inflammatory cytokines in NCI-H292 cells. These findings suggest that the ethanol extract of Platycodon grandiflorum leaves containing luteolin-7-O-glucoside exhibits promising anti-inflammatory properties.

서 론
재료 및 방법
    1. 도라지 잎 추출물
    2. 지표성분 확인 및 함량 측정
    3. 세포배양
    4. 세포 생존율 측정
    5. Nitrite 및 cytokine 측정
    6. 전기영동 및 western blot 분석
    7. 통계처리
결과 및 고찰
    1. 도라지 잎 에탄올 추출물의 지표성분 함량분석
    2. 마우스 유래 대식세포에서 에탄올 추출물의 항염작용
    3. 인체 유래 폐암세포에서 에탄올 추출물의 항염작용
요약 및 결론
References

• 이정민(호서대학교 대학원 생명공학과 대학원생) | Jung Min Lee (Master’s Student, Dept. of Biotechnology, Graduate School, Hoseo University, Asan 31499, Korea)
• 배병준(호서대학교 대학원 생명공학과 대학원생) | Byeong Jun Bae (Master’s Student, Dept. of Biotechnology, Graduate School, Hoseo University, Asan 31499, Korea)
• 최지림((주)황초원 대표이사) | Jee-Lim Choi (CEO, Royal Greens Co., Ltd., Seongnam 13201, Korea)
• 정영신(호서대학교 생명공학과 교수, 호서대학교 기초과학연구소 교수) | Young-Shin Chung (Professor, Dept. of Biotechnology, Hoseo University, Asan 31499, Korea, Professor, The Research Institute for Basic Sciences, Hoseo University, Asan 31499, Korea) Corresponding author