This study focused on the genomic analysis of Anopheles kleini and Anopheles pullus, both vectors of vivax malaria within the Anopheles Hyrcanus group. Using Illumina NovaSeq600 and Oxford Nanopore platforms, we identified 126 and 116 contigs, along with 40,420 and 32,749 genes from An. kleini and An. pullus, respectively. The assembled genome sizes were 282 Mb for An. kleini and 247 Mb for An. pullus, which are within a similar range to the sizes previously estimated by digital PCR (249 Mb and 226 Mb). We are currently also estimating the genome sizes of other Anopheles spp. and manually curating key genes determining vectorial capacity.

• Do Eun Lee(Department of Tropical Medicine and Parasitology, Seoul National University)
• Jeong Heum Han(Department of Tropical Medicine and Parasitology, Seoul National University)
• Il Hwan Kim(Department of Chemical and Biological Metrology, Korea Research Institute of Standards and Science)
• Jongsun Park(Infoboss Inc., 301 room, Gaeun Bldg., 670, Seolleung-ro, Gangnam-gu, Seoul)
• Ju Hyeon Kim(Department of Tropical Medicine and Parasitology, Seoul National University)