An unrecorded gall midge was discovered from inflorescence galls on Castanopsis sieboldii (Makino) Hatus. ex T.Yamaz. & Mashiba, which is one of major components in evergreen forests on the Korean Peninsula. The galls occurred on 20 trees out of 230 on Yokiji Island. The gall midge was identified as Schizomyia castanopsisae Elsayed & Tokuda, 2018 (Diptera: Cecidomyiidae), using morphological characters and mitochondrial DNA cytochrome oxidase subunit 1 (COI) region sequences of gall midge’s larvae. The Barcode sequences of 40 samples collected from Yokji Island were identical, and the individuals from Yokji Island formed a clade with the individuals from Kyushu, with robust bootstrap support in a maximum likelihood tree. This result suggests the gall midges may have migrated from Kyushu, Japan to Yokji Island, South Korea. However, it is too early to determine if the gall midge is truly invasive or not at present due to paucity of distribution data in the country.