Stoneflies (Plecoptera) are known for being sensitive to water pollution and are used as bioindicators for evaluating water quality. Among them, Nemouridae, especially the genus Nemoura, which are commonly referred to as winter stoneflies, can be found around streams even during the cold winter months. Nemoura geei Wu, 1929, among them, was originally described from Beijing and is widely distributed in Korea, China, Japan, and the Russian Far East. Here, we report the development and characterization of new functional microsatellite markers of N. geei using high-throughput sequencing technology. A total of 80,661 microsatellite loci were identified with a total length of 1,801,591 bp. The average length was 22.34 bp, and microsatellites occupied 0.42% of the entire sequence. The novel 20 microsatellite markers developed in this study can be usefully applied to the population genetics analyses as important genetic resources for understanding the ecological and evolutionary characteristics of a stonefly species at the population level in Korea.

• Ji Hyoun Kang(Department Korean Entomological Institute, Korea University, Seoul, Korea)
• Jun Mi Hur(National Institute of Biological Resources, Incheon, Korea)
• Yeon Jae Bae(Department Korean Entomological Institute, Korea University, Seoul, Korea, Division of Environmental Science and Ecological Engineering, Korea University, Seoul, Korea)
• Jeong Mi Hwang(Department Korean Entomological Institute, Korea University, Seoul, Korea)