In insects, the glutathione S-transferase is initiated in both the detoxification process and the protection of cellular membranes against oxidative damage. In this study, we identified the open reading frame (ORF) sequence of GST-iso1 and 2 from Tenebrio molitor (TmGST-iso1 and 2). To investigate the expression patterrns of TmGST-iso1 and 2 in response to herbicide, 0.06, 0.6, and 6 ㎍/㎕ of butachlor (FarmHannong, Seoul, South Korea) was challenged into T. molitor larvae, resulting that the TmGST-iso1 were highly induced at 3 and 24 h-post injection. Whereas, the highest expression of TmGST-iso2 was detected at 24 h after treatment. This study may contribute to basic information about the detoxifying activities of T. molitor.

• Seo Jin Lee(Department of Biology, College of Natural Sciences, Soonchunhyang University, Korea Native Animal Resources Utilization Convergence Research Institute (KNAR), Soonchunhyang University)
• Ho Am Jang(Department of Biology, College of Natural Sciences, Soonchunhyang University, Korea Native Animal Resources Utilization Convergence Research Institute (KNAR), Soonchunhyang University)
• Sung Min Ku(Department of Biology, College of Natural Sciences, Soonchunhyang University, Korea Native Animal Resources Utilization Convergence Research Institute (KNAR), Soonchunhyang University)
• Jea Hui Kim(Department of Biology, College of Natural Sciences, Soonchunhyang University, Korea Native Animal Resources Utilization Convergence Research Institute (KNAR), Soonchunhyang University)
• Dong Woo Kang(Department of Biology, College of Natural Sciences, Soonchunhyang University)
• So Yeon Choi(Department of Biology, College of Natural Sciences, Soonchunhyang University)
• Yong Seok Lee(Department of Biology, College of Natural Sciences, Soonchunhyang University, Korea Native Animal Resources Utilization Convergence Research Institute (KNAR), Soonchunhyang University)
• Yeon Soo Han(Department of Applied Biology, College of Agriculture and Life Sciences, Chonnam National University)
• Yong Hun Jo(Department of Biology, College of Natural Sciences, Soonchunhyang University)