작은소피참진드기(Haemaphysalis longicornis)는 중증열성혈소판감소증후군 (SFTS)을 전파하는 주요 매개 체로 알려져 있으며, 대한민국 전역에 분포하고 있다. SFTS는 2013년 첫 환자 보고 이후 SFTS의 보고 사례가 꾸준히 증가하고 있으며 이에 해당 질병을 전파하는 주요 매개체인 작은소피참진드기를 대상으로 한 연구의 중요성이 증가하고 있다. 전사체 분석은 llumina HiSeq 4000 platform을 활용하여 진행하였다. Illumina HiSeq 4000에서 확보된 전사체 데이터를 Trinity 프로그램을 활용하여 de novo assembly를 진행하였으며, 이후 TGICL 프로그램을 통해 unigenes 을 확보하였다. 이후 확보된 unigenes는 전사체의 기능을 추정 및 식별하기 위해 Swiss-Prot, KOG, GO, KEGG, PANM DB를 기반으로 한 BLASTx를 수행하였다. 또한 Phlebovirus의 존재 여부를 확인하기 위해 NCBI에 등록된 Phlebovirus 유전자원을 수집하여 데이터베이스를 구축하였으며, 구축된 데이터베이스에서 BLASTx를 진행하 여 바이러스 전사체의 존재 여부를 분석하였다. 확보된 28,078개의 unigenes 중 19,414개의 unigene이 PANM DB에 annotation 되었으며, Swiss-Prot, KOG, GO, KEGG에서는 각각 13,117개, 13,002개, 8,588개, 1,651개의 unigene이 annotation 되었다. Phlebovirus 전사체 존재 여부 분석 결과, BLASTX를 통해 작은소피참진드기로부터 SFTS RNA polymerase와 유사성을 보이는 3개의 unigene의 존재를 확인하였다. 이번 연구 결과는 국내 작은소피참진드기 및 SFTS 바이러스 감시와 전염병 예방·통제에 있어 기초 자료로써 활용될 수 있을 것으로 사료된다.

• Hyeok Lee(Department of Biology, College of Natural Sciences, Soonchunhyang University, Korea Native Animal Resources Utilization Convergence Research Institute (KNAR))
• Jie Eun Park(Korea Native Animal Resources Utilization Convergence Research Institute (KNAR), Research Support Center (Core-Facility) for Bio-Bigdata Analysis and Utilization of Biological Resources)
• Min Kyu Sang(Korea Native Animal Resources Utilization Convergence Research Institute (KNAR), Research Support Center (Core-Facility) for Bio-Bigdata Analysis and Utilization of Biological Resources)
• Bharat Bhusan Patnaik(Department of Biology, College of Natural Sciences, Soonchunhyang University, Korea Native Animal Resources Utilization Convergence Research Institute (KNAR), PG Department of Biosciences and Biotechnology, Fakir Mohan University, India)
• Dae Kwon Song(Department of Biology, College of Natural Sciences, Soonchunhyang University, Korea Native Animal Resources Utilization Convergence Research Institute (KNAR), Research Support Center (Core-Facility) for Bio-Bigdata Analysis and Utilization of Biological Resources)
• Jun Yang Jeong(Department of Biology, College of Natural Sciences, Soonchunhyang University, Korea Native Animal Resources Utilization Convergence Research Institute (KNAR))
• Chan Eui Hong(Department of Biology, College of Natural Sciences, Soonchunhyang University, Korea Native Animal Resources Utilization Convergence Research Institute (KNAR))
• Chan Eui Hong(Department of Biology, College of Natural Sciences, Soonchunhyang University, Korea Native Animal Resources Utilization Convergence Research Institute (KNAR))
• Hyeon Jun Shin(Department of Biology, College of Natural Sciences, Soonchunhyang University, Korea Native Animal Resources Utilization Convergence Research Institute (KNAR)Department of Biology, College of Natural Sciences, Soonchunhyang University, Korea Native Animal Resources Utilization Convergence Research Institute (KNAR))
• Liu Ziwei(Department of Biology, College of Natural Sciences, Soonchunhyang University, Korea Native Animal Resources Utilization Convergence Research Institute (KNAR))
• Hee Ju Hwang(Department of Biology, College of Natural Sciences, Soonchunhyang University, Korea Native Animal Resources Utilization Convergence Research Institute (KNAR))
• So Young Park(Biodiversity Research Team, Animal & Plant Research Department, Nakdonggang National Institute of Biological Resources)
• Se Won Kang(Biological Resource Center (BRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB), Korea)
• Hang Chul Cho(DSC (Data Science Center) INSILICOGEN, INC.)
• Heon Cheon Jeong(Korea Native Animal Resources Utilization Convergence Research Institute (KNAR))
• Hong Seog Park(Research Institute, GnC BIO Co., LTD.)
• Hongray Howrelia Patnaik(PG Department of Zoology, BJB Autonomous College, Odisha)
• E Hyun Shin(Department of Biology, College of Natural Sciences, Soonchunhyang University)
• Sung-Jae Cha(Johns Hopkins Bloomberg School of Public Health, Department of Molecular Microbiology and Immunology and Malaria Research Institute, USA)
• Yong Hun Jo(Department of Biology, College of Natural Sciences, Soonchunhyang University, Korea Native Animal Resources Utilization Convergence Research Institute (KNAR))
• Yeon Soo Han(College of Agriculture and Life Science, Chonnam National University, Korea.)
• Yong Seok Lee(Department of Biology, College of Natural Sciences, Soonchunhyang University, Korea Native Animal Resources Utilization Convergence Research Institute (KNAR), Research Support Center (Core-Facility) for Bio-Bigdata Analysis and Utilization of Biological Resources)