A lipase inhibitor from Desmodium oxyphyllum DC. was purified by methanol extraction, systematic solvent extraction, silica gel column chromatography, C18 solid phase extraction chromatography and RP-HPLC. We obtained the purified lipase inhibitor with 182 ng(IC50) of lipase inhibitory activity for a 0.06% yield. Its molecular weight was estimated to be 655.37 Da from an instrumental analysis of MALDI-TOF-MS and it was identified copper-3, 5-dibromo-2-hydroxybenzoic acid (C14H8Br4CuO6) by 1H, 13C NMR analysis.

• 곽연주 | Yeon Ju Kwak